标题: 使用氨氮替换生长猪日粮中的非必需氨基酸不会影响胴体沉积蛋白的氨基酸组成 [打印本页] 作者: IMCTT 时间: 2017-10-30 15:45 标题: 使用氨氮替换生长猪日粮中的非必需氨基酸不会影响胴体沉积蛋白的氨基酸组成 2017. J. Anim. Sci. 95(10): 4481-4489 使用氨氮替换生长猪日粮中的非必需氨基酸不会影响胴体沉积蛋白的氨基酸组成
W. D. Mansilla, J. K. Htoo and C. F. M. de Lange
Replacing dietary non essential amino acids with ammonia nitrogen does not alter amino acid profile of deposited protein in the carcass of growing pigs fed a diet deficient in non essential amino acid nitrogen
W. D. Mansilla, J. K. Htoo and C. F. M. de Lange
Amino acid usage for protein retention, and, consequently, the AA profile of retained protein, is the main factor for determining AA requirements in growing animals. The objective of the present study was to determine the effect of supplementing ammonia N on whole-body N retention and the AA profile of retained protein in growing pigs fed a diet deficient in nonessential AA (NEAA) N. In total, 48 barrows with a mean initial BW of 13.6 kg (SD 0.7) were used. At the beginning of the study, 8 pigs were euthanized for determination of initial protein mass. The remaining animals were individually housed and fed 1 of 5 dietary treatments. A common basal diet (95% of experimental diets) was formulated to meet the requirements for all essential AA (EAA) but to be deficient in NEAA N (CP = 8.01%). The basal diet was supplemented (5%) with corn starch (negative control) or 2 N sources (ammonia or NEAA) at 2 levels each to supply 1.35 or 2.70% extra CP. The final standardized ileal digestible (SID) NEAA content in the high-NEAA-supplemented diet (positive control) was based on the NEAA profile of whole-body protein of 20-kg pigs, and it was expected to reduce the endogenous synthesis of NEAA. Pigs were fed at 3.0 times maintenance energy requirements for ME in 3 equal meals daily. At the end of a 3-wk period, pigs were euthanized and the carcass and visceral organs were weighed, frozen, and ground for determination of protein mass. From pigs in the initial, negative control, high-ammonia, and high-NEAA groups, AA contents in the carcass and pooled visceral organs were analyzed to determine the total and deposited protein AA profile, dietary EAA efficiencies, and minimal de novo synthesis of NEAA. Carcass weight and whole-body N retention linearly increased (P 0.10) between N sources, but Cys content increased (P 0.10) between N sources. The de novo synthesis of NEAA increased (P
翻译:朱滔 猪营养国际论坛CSIS